A gram stain of urethral discharge is a test used to identify bacteria in fluid from the tube that drains urine from the bladder (urethra).
Urethral discharge Gram stain
How the test is performed
Fluid from the urethra is collected on a cotton swab. A sample from this swab is applied in a very thin layer to a microscope slide. A series of stains called a gram stain is applied to the specimen.
The stained smear is then examined under the microscope for the presence of bacteria. The color, size, and shape of the cells help identify the organism causing the infection.
How to prepare for the test
This test is often performed in the health care provider's office.
How the test will feel
You may feel pressure or burning when the cotton swab touches the urethra.
Why the test is performed
The test is performed when an abnormal urethral discharge is present. It may be performed if a sexually transmitted disease is suspected.
Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your doctor about the meaning of your specific test results.
What abnormal results mean
Abnormal results may indicate gonorrhea or other infections.
What the risks are
There are no risks.
A culture of the specimen (urethral discharge culture) should be performed in addition to the gram stain. More sophisticated diagnostic tests (such as PCR tests) are sometimes also done.
McCormack WM. Urethritis. In: Mandell GL, Bennett JE, Dolin R, eds. Principles and Practice of Infectious Diseases. 7th ed. Philadelphia, Pa: Elsevier Churchill Livingstone; 2009:chap 106.
Cohen MS. Approach to the patient with a sexually transmitted disease. In: Goldman L, Ausiello D, eds. Cecil Medicine. 24th ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 293.
Linda J. Vorvick, MD, Medical Director, MEDEX Northwest Division of Physician Assistant Studies, University of Washington, School of Medicine. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M., Inc.